High-throughput fluorescence detection using an integrated zone-plate array
نویسندگان
چکیده
منابع مشابه
High-throughput fluorescence detection using an integrated zone-plate array.
Microfluidic devices enable massive parallelization of sample manipulation and delivery, but a similarly parallelized and integrated optical detection system does not yet exist. Standard large numerical aperture wide field or scanning optical systems are not capable of the large field of view and detection sensitivity required to collect fluorescence from parallel arrays of microfluidic devices...
متن کاملZone-plate-array lithography using synchrotron radiation
We present our results in the design and development of a multiple-beam x-ray pattern generator based on an array of Fresnel zone plates ~FZPs!. Exposures have been carried out using a synchrotron source at SuperACO ~France!, a PC-controlled piezoelectric scanning stage, and an array of FZPs fabricated by high resolution electron-beam lithography and reactive-ion etching of a tungsten absorber....
متن کاملZone-plate-array Lithography (zpal)
Nanolithography is the key technology driving technological progress in electronics, photonics, information technology and biotechnology. The tools and techniques used in the semiconductor industry have become too expensive for applications other than high-volume manufacturing. Masks, which are required for each unique design, can cost tens of thousands of dollars per layer. This economic const...
متن کاملHigh-throughput single-molecule fluorescence spectroscopy using parallel detection.
Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. The basic concept of this technique is to excite and collect light from a very small volume (typically femtoliter) and work in a concentration regime resulting in rare burst-like events corresponding to the transit of a single-molecule. Those even...
متن کاملFluorescence lifetime plate reader: resolution and precision meet high-throughput.
We describe a nanosecond time-resolved fluorescence spectrometer that acquires fluorescence decay waveforms from each well of a 384-well microplate in 3 min with signal-to-noise exceeding 400 using direct waveform recording. The instrument combines high-energy pulsed laser sources (5-10 kHz repetition rate) with a photomultiplier and high-speed digitizer (1 GHz) to record a fluorescence decay w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2010
ISSN: 1473-0197,1473-0189
DOI: 10.1039/b923554j